Polysaccharide from Fruits of Physalis Alkekengi L. Enhances Antitumor Efficacy by a DNA Vaccine

Updata:01-01-1970

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By:Yun Xi, Donglin Zhu, Jing Huang, Jingping Liu, Hua Li, Gang Xiao

DOI: https://doi.org/10.30564/jams.v1i4.291

Abstract:

 Physalis. alkekengi fruit has long been used in traditional Chinese medicine for tumor therapy. In the present study, using plasmids that encode ovalbumin (OVA) gene, we investigate the adjuvant activity of a polysaccharide fraction (PPSB) isolated from P.alkekengi fruit. Formulation by simple procedures of mixing of the OVA-encoding pCI-neo-sOVA plasmid with PPSB not only induced specific antibody responses, but also induced antigen-specific cytotoxic T lymphocyte (CTL) responses (Graph abstract). Furthermore, immunization using this vaccine prevented the growth of OVA-expressing B16-OVA tumor cell growth in the immunized mice. Thus, we provide evidence supporting the adjuvant activity of PPSB in DNA vaccine against tumor.

References:

[1] A. Stachyra, A. Gora-Sochacka, A. Sirko. DNA vaccines against influenza[J]. Acta biochimica Polonica, 2014(61):515-522. [2] J.R. Greenland, N.L. Letvin. Chemical adjuvants for plasmid DNA vaccines[J]. Vaccine, 2007(25):3731-3741. [3] J. Zhou, & Wang, L. Analysis of nutrient composition in the fruit of Physalis alkekengi L[J]. Journal of Nutrition, 1997(19):243-245. [4] H. Yang, S. Han, D. Zhao, G. Wang. Adjuvant effect of polysaccharide from fruits of Physalis alkekengi L. in DNA vaccine against systemic candidiasis[J]. Carbohydr Polym, 2014(109):77-84. [5] C.P. Committee. Pharmacopoeia of The People’s Republic of China[M]. China Medical Science Press, Beijing,2010. [6] H. Tong, Liang, Z., & Wang, G. Structural characterization and hypoglycemicactivity of a polysaccharide isolated from the fruit of Physalis alkekengi L[J]. Carbo-hydrate Polymers, 2008(71):316-323. [7] G. Xiao, X. Li, A. Kumar, Z. Cui. Transcutaneous DNA immunization following waxing-based hair depilation elicits both humoral and cellular immune responses, European journal of pharmaceutics and biopharmaceutics[J].Official Journal of Arbeitsgemeinschaft fur Pharmazeutische Verfahrenstechnik e.V, 2012(82):212-217. [8] R. Toll, U. Jacobi, H. Richter, J. Lademann, H. Schaefer, U. Blume-Peytavi, Penetration profile of microspheres in follicular targeting of terminal hair follicles[J]. The Journal of Investigative Dermatology, 2004(123):168-176. [9] G. Chaouat. The Th1/Th2 paradigm: still important in pregnancy?[C]. Seminars in Immunopathology, 2007(29):95-113. [10] J. Pol, N. Bloy, F. Obrist, A. Eggermont, J. Galon, W. Herve Fridman, I. Cremer, L. Zitvogel, G. Kroemer, L. Galluzzi. Trial Watch: DNA vaccines for cancer therapy[J]. Oncoimmunology, 2014(3):e28185. [11] S.G. Reed, S. Bertholet, R.N. Coler, M. Friede. New horizons in adjuvants for vaccine development[J]. Trends in Immunology, 2009(30):23-32. [12] S.G. Reed, M.T. Orr, C.B. Fox. Key roles of adjuvants in modern vaccines[J]. Nature Medicine, 2013(19):1597-1608. [13] S.P. Smeekens, M.S. Gresnigt, K.L. Becker, S.C. Cheng, S.A. Netea, L. Jacobs, T. Jansen, F.L. van de Veerdonk, D.L. Williams, L.A. Joosten, C.A. Dinarello, M.G. Netea. An anti-inflammatory property of Candida albicans beta-glucan: Induction of high levels of interleukin-1 receptor antagonist via a Dectin-1/CR3 independent mechanism[J]. Cytokine, 2014(71):215-222. [14] D. Gordon, P. Kelley, S. Heinzel, P. Cooper, N. Petrovsky. Immunogenicity and safety of Advax, a novel polysaccharide adjuvant based on delta inulin, when formulated with hepatitis B surface antigen: A randomized controlled Phase 1 study[J]. Vaccine, 2014(32):6469-6477. [15] P.s.C.o.A.o.S.a. Technology, Report to the President on reengineering the influenza vaccine production enterprise to meet the challenges of pandemic influenza[EB/OL].http://www.whitehouse.gov/sites/default/files/microsites/ostp/PCAST-Influenza-Vaccinology-Report.pdf, 2010.

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