Photosynthesis of Submerged and Surface Leaves of the Dwarf Water Lily (Nymphoides aquatica) Using PAM Fluorometry

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DOI: https://doi.org/10.30564/jbr.v4i3.4820

Abstract:

Dwarf Water Lilies (Nymphoides aquatica (J.F. Gmel) Kuntze have floating and submerged leaves. Some submerged aquatic vascular plants have a form of CAM (Crassulacean Acid Metabolism) called Submerged Aquatic Macrophyte (SAM) metabolism. Blue-diode based PAM technology was used to measure the Photosynthetic Oxygen Evolution Rate (POER: 1O₂ ≡ 4e^– ). Optimum Irradiance (E_opt), maximum POER (POER_max) and quantum efficiency (α₀) all vary on a diurnal cycle. The shape of the POER vs. E curves is different in seedling, submerged and surface leaves. Both E_opt and POER_max are very low in seedling leaves (E_opt ≈ 104 μmol photon m^–2 s^–1, PPFD; POER_max ≈ 4.95 µmol O₂ g–1 Chl a s^–1), intermediate in mature submerged leaves (E_opt ≈ 419 µmol photon m^–2 s^–1 PPFD, POER_max ≈ 38.1 µmol O₂ g^–1 Chl a s^–1) and very high in surface leaves (E_opt ≈ 923 µmol photon m^–2 s^–1 PPFD, POER_max ≈ 76.1 µmol O₂ g^–1 Chl a s^–1). Leaf titratable acid (C4 acid pool) is too small (≈20 to 50 mol H⁺ m^–3) to support substantial SAM metabolism. Gross daily photosynthesis of surface leaves is ≈3.71 g C m^–2 d^–1 in full sun and as much as 1.4 gC m^–2 d^–1 in shaded submerged leaves. There is midday inhibition of photosynthesis.

References:

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