Piscicidal Effects of Terminalia arjuna Leaf, Bark and Fruit Extract on a Fresh Water Predatory Catfish, Heteropneustes fossilis

Source:
By:Suely Akter, Hossain M. Zabed, Munira Nasiruddin, Xianghui Qi

DOI: https://doi.org/10.30564/re.v2i1.1328

Abstract:

Piscicidal effects of several solvent extarcts (distilled water, 50% ethanol, absolute ethanol and 80% methanol) of Terminalia arjuna leaves, barks and fruits were studied against a common fresh water predatory fish, Heteropneustes fossilis under laboratory conditions in terms of Behavior and mortality of fishes after 24 h. Fishes exposed to plant extracts showed agitating movement with quick surfacing, loss of balance, mucus secretion, and finally died. The LC₅₀ values of distilled water, 50% ethanol, absolute ethanol and 80% methanol extracts were found to be 311.726, 236.141, 183.541, 478.794 ppm for leaves, 117.894, 96.998, 38.990, 304.193 ppm for barks and 1400.033, 949.209, 555.201, 875.158 ppm for fruits, respectively. Chi-square values were found to be insignificant at P<0.05 in almost all plant extracts, indicating that observed and expected mortalities did not vary significantly in relation to doses, except 80% methanol extract of barks and absolute ethanol extract of fruits. The F-values of treatments were significant at P<0.01, except 80% methanol extract of fruits, in which F-values were insignificant in all replicates at P>0.01. Based on LC₅₀ values, order of piscicidal activity followed the pattern, bark > leaf > fruit extracts. Order of piscicidal activity for the extracts was like absolute ethanol > 50% ethanol > distilled water > 80% methanol for leaf and bark, while for fruit extracts, trend was like absolute ethanol > 80% methanol > 50% ethanol > distilled water.

References:

[1] Singh D, Singh A. Piscicidal effect of some common plants of India commonly used in fresh water bodies against target animals. Chemosphere, 2002, 49: 45-49. [2] Olaifa F E, Hamzat R A, Oyetoyan O O. Acute toxicity of ethanol extracts of cocoa bean shell on Sarotherodon galilaeus juveniles. J Fish Int, 2008, 3: 56-60. [3] Baskaram P, Palanichamy S, Visalakshi S, et al. Effects of mineral fertilizers on survival of the fish Oreochromis mossambicus. Environ Ecol, 1989, 7: 463-465. [4] Kalavanthy K, Sivakumar A A, Chandran R. 2001. Toxic effect of the pesticide dimethoate on the fish Sarotherodon mossambicus. J Ecol Res Bioconserv, 2001, 2: 27-32. [5] Santharan K R, Thayunmanavum B, Krishnaswamy S. Toxicity of some insecticides to Daphnia carinata King and important link in the food chain in fresh ecosystem. Indian J Ecol, 1975, 3: 70-73. [6] Pant P C, Singh T. Inducement of metabolic dysfunction by carbomate and organophosphorus compounds in a fish, Puntius conchonius. Pestle Biochem Physiol, 1983, 20: 294-298. [7] Hodson P V. The effect of metal metabolism on uptake, disposition and toxicity in fish. Aquat Toxicol, 1988, 11: 3-18. [8] Johl M S, Dua A. Elemental lepidological and toxicological studies in Channa punctatus (Block) upon exposure to an organochlorine pesticide, endosulfane. Bull Environ Contam Toxicol, 1995, 55: 916-921. [9] Suely A., Zabed H., Ahmed A.B.A., Mohamad J., Nasiruddin M., Sahu J.N., Ganesan P.. Toxicological and hematological effect of Terminalia arjuna bark extract on a freshwater catfish, Heteropneustes fossilis. Fish Physiol Biochem, 2016, 42: 431–444. [10] Sudhanshu T, Singh A.. Piscicidal activity of alcoholic extract of nerium indicum leaf and their biochemical stress response on fish metabolism. Afr J Trad Med, 2004, 1: 15-29. [11] Kumar A, Prasad M R, Srivastava K, et al.. Branchial histopathological study of catfish Heteropneustes fossilis following exposure to purified neem extract, Azadirachtin. World J Zool, 2010, 5: 239-243. [12] Burkil H N. The useful plants of west tropical africa, 2nd Ed, Vol 1, Families A-D, Royal Botanical Garden, Kew, 1985. [13] Wang S, Huffman J B.. Botanochemicals: Supplements to petrochemicals. Econ Bot, 1991, 35: 369-382. [14] Fayez E K, Mahmoud I N. A tannin anti-cancer promotor from Terminalia arjuna. Phytochem, 1998, 47: 1567-1568. [15] Tripathi S M, Singh D K. Molluscicidal activity of Punica granatum bark and Canna indica root. Braz J Med Bio Res, 2000, 33: 1351-1355. [16] Rao I G, Singh A, Singh V K, et al. Effect of single and binary combinations plant-derived molluscicides on different enzyme activities in the nervous tissue of Achatina fulica. J App Toxicol, 2003, 23: 19-22. [17] Prajapati V, Tripath A. K, Khanuja S P S, et al. Anti-insect screening of medicinal plants from Kukrail forest, Lucknow, India. Pharmaceut Biol, 2003, 41: 166-170. [18] Neuwinger H D. Plants used for poison fishing in tropical Africa. Toxicon, 2004, 44: 417-430. [19] Singh A, Singh S K. Molluscicidal evalution of three common plants from India. Fitoterapia, 2005, 76: 747-751. [20] Latifa G A, Hamid A, Sharma G.. Piscicidal activity of dry bark of Diospyros ebenum (koen) on H. fossilis (Bloch) and Anabas testudineus (Bloch). Bang J Life Sci, 2002, 14: 31-36. [21] Latifa G A, Begum M T, Bachar S C. Piscicidal activity of dry barks of Leucaena leucocephala (Lam. De Wit) on Channa. punctatus (Bloch) and Channa striatus (Bloch). Bangladesh J Zool, 2004, 32: 247-251. [22] Nasiruddin M, Azadi M A, Sultana N. Histopathological effects of dry seed extracts of four medicinal plants on Heteropneustes fossilis (Bloch). Chitt Univ J B Sci, 2007, 2: 113-126. [23] Nasiruddin M, Azadi M A, Rahman I A S. Toxicological effect of Acacia auriculaeformes (A. Cunn. Ex. Bench) and Mesua ferrea (L.) plant parts on Heteropneustes fossilis (Bloch). Bangladesh J Zool, 2009, 37: 103-112. [24] American Public Health Association (APHA). Standard methods for the examination of water and waste water. APHA Press, Washington DC, 1976. [25] American Public Health Association (APHA). Standard methods for the examination of water and waste water. APHA Press, Washington DC, 1998. [26] Finney D J. Probit analysis, 3rd ed. Cambridge University Press, London, 1971. [27] Fisher R A, Yates F. 1963. Statistical tables for biological, agricultural and medicinal research, 6th ed. Oliver and Boyd Ltd, Edinburgh, 1963: 47-50. [28] Chowdhury S M. 1969. Introduction to statistical theory, part 11. Parch Made Industries and Press, Lahore, Pakistan, 1969. [29] Latifa G A, Begum A. Piscicidal activity of the dry stem of Euphorbia neriifolia (Lin. 1753) on Heteropneustes fossilis (Bloch) and Channa punctatus (Bloch). Bangladesh J Sci Res, 1993, 11: 217–225. [30] Wedemeyer G. 1970. Stress of anaesthesia with MS-222 and benzocaine in rainbow trout (Salmo gairdneri). J Fish Bed Con, 1970, 27: 909-914. [31] Sharma A P, Al-Nasiri S K, Bhatt M N. 1978. Toxicity and efficacy of MS-222 on three fishes in Iraq. Bangladesh J Zool, 1978, 6: 107-111. [32] Chakraborty M. Piscicidal, histopathological and heamolytic effects of fruit, bark and leaf extracts of Terminalia chebula (Retz) on H. fossilis (Bloch). Dessertation for Master Degree. Department of Zoology, University of Chittagong, 2005. [33] Khalil M I. 1984. Study of the piscicidal property of the indigenous Sapium indicum fruits (Fam: Euphorbiaceae). Dessertation for Master Degree. Department of Zoology, University of Dhaka, 1984. [34] Zbinden G, Flury-Roversi M. 1981. Significance of the LC50 test for the toxicological evaluation of chemical substance. Arch Toxicol, 1981, 47: 79-99. [35] Wallance-Hayes A. Principles and methods of toxicology. Raven press, New York, 1982. [36] Zabed H., Suely A., Faruq G., Sahu J.N.. Water quality assessment of an unusual ritual well in Bangladesh and impact of mass bathing on this quality. Sci Total Environ, 2014, 472: 363-369. [37] Ameen M, Chowdhury A K A, Khan H R, et al. Insecticidal propertie of D. elliptica (wall) roots against the larvae of Culex fatigans (Diptera: Culicidae). Dhaka Univ Stud B, 1983, 31: 1–11. [38] Latifa G A, Shafi M, Pervin S I et al. Piscicidal property of dry root of Tephrosia purpurea (Pers) on H. fossilis (Bloch) and C. punctatus (Bloch). J Asiat Soc. Bangladesh Sci, 1988, 14: 48-50.