Surveillance of Vibrio spp. in Penaeus monodon Collected from Shrimp Pond of Satkhira, Bangladesh

Source:
By:Shahena Aktar Shipa

DOI: https://doi.org/10.30564/jzr.v2i3.2087

Abstract:

Vibrio is the most common genera associated with crustaceans and often causing significant economic losses. Many Vibrio species are pathogenic to human and have been implicated in food borne diseases. The present study was conducted to identify Vibrio spp. from the tiger shrimp (Penaeus monodon) of shrimp pond at Satkhira, Bangladesh. A total number of 33 Vibrio species isolates were identified from 20 shrimp samples through a series of morphological, physiological and biochemical tests. The work reports the prevalence of Vibrio spp. in the pond environments and the existence of three Vibrio species such as V. alginolyticus, V. parahaemolyticus and V. harveyi were identified. In the study of antibiogram, all isolates were shown 100% sensitive to streptomycin, ciprofloxacin and chloramphenicol. Maximum 41% isolates were shown resistant to co-trimethaxozole whereas 30% and 24% resistant to azithromycin and novobiocin respectively.

References:

[1] Karim, M.F., Zhang, X. and Li, R. (2019) Dynamics of Shrimp Farming in the SouthwesternCoastal Districts of Bangladesh Using a Shrimp YieldDataset (SYD) and Landsat Satellite Archives. Sustainability 11:4635, doi:10.3390/su11174635. [2] BBS (Bangladesh Bureau of Statistics). Bangladesh Statistics 2017, Ministryof Planning: Dhaka, Bangladesh. [3] Ahasan, D.A. (2012) Bangladesh's Winning Maritime Boundary, Development, Conservation and Management of Fish Resources, National Fish Week 2012 Compendum (in Bengali), Narinda, Dhaka: Department of Fisheries, Ministry of Fisheries and Livestock, GoB., 23–25. [4] Rahman, M.M. and Hossain, M.M. (2009) Production and export of shrimp of Bangladesh: Problems and prospects. Progressive Agriculture 20(1, 2): 163–171. [5] BER (2009 and 2010) Bangladesh Economic Review (BER) 2007. Economic Adviser’s Wing. Finance Division, Ministry of Finance. Government of the People’s Republic of Bangladesh. [6] Lightner, D.V. (1993) Diseases of cultured penaeid shrimp. (Eds. McVey, J.P.) CRC Handbook of Mariculture, 2nd ed. Crustacean Aquaculture, vol. 1. CRC Press, Boca Raton FL, 393– 486. [7] Rahman, S., Khan, S.N., Naser, M.N. and Karim, M.M. (2010) Isolation of Vibrio spp. from Penaeid Shrimp Hatcheries and Coastal Waters of Cox’s Bazar, Bangladesh.ASIAN J. EXP. BIOL. SCI. 1 (2): 288 - 293 [8] Petersen, J. and Mclaughlin, S. (2016) Laboratory Exercises in Microbiology: Discovering the Unseen World Through Hands-On Investigation. City University of New York (CUNY) CUNY Academic Works. [9] Chakma, F., Sabuj, A.A.M., Haque, Z.F., Pondit, A.,Talukder, B., Kabir, S.M.L. and Saha, S. (2018) Isolation and characterization of Vibrio sp from semi processed shrimp. Journal of Research in Biology 8(7): 2566-2573 [10] Teng, T., Liang, L., Chen, K., Xi, B., Xie, J. and Xu, P. (2017) Isolation, identification and phenotypic and molecular characterization of pathogenic Vibrio vulnificus isolated from Litopenaeus vannamei. PLoS ONE 12(10): e0186135, https://doi.org/10.1371/journal.pone.0186135 [11] Pedersen, K., Tiainen, T. and Larsen, J.L. (1995) Antibiotic resistance of Vibrio anguilarum, in relation to serovar and plasmid contents. Acta vet.scand 36: 55-64. [12] Austin, B. and Austin, D.A. (1993) Bacterial fish pathogens, Diseases in farmed and wild fish, 2nd edn. Ellis Horwood Ltd., Chichester. [13] Lee, K.K., Yu, S.R., Chen, F.R., Yang, T.I. and Liu, P.C. (1996) Virulence of Vibrio alginolyticus isolated from diseased tiger prawn, Penaeus monodon. Curr. Microbiol. 32: 229-231, DOI: 10.1007/s002849900041 [14] Mercedes, A. and Anicet, R.B. (1994) A set of keys for biochemical identification of environmental Vibrio species. Journal of Applied Bacteriology 76: 79-85. [15] Sung, H.H., Hsu, S.F., Chen, C.K., Ting, Y.Y. and Chao, W.L. (2001) Relationships between disease outbreak in cultured tiger shrimp (Penaeus monodon) and the composition of Vibrio communities in pond water and shrimp hepatopancreas during cultivation. Aquaculture 192: 101-110, DOI: 10.1016/s0044-8486(00)00458-0 [16] Choopun, N., Louis, V., Haq, A. and Colwell, R.R. (2002) Simple Procedure for Rapid Identification of Vibrio cholerae from the Aquatic Environment. Applied and Environment Microbiology 68(2): 995-998. [17] Alsina, M. and Blanch, A.R. (1994) A set of keys for biochemical identification of environmental Vibrio species. J. Appl. Bacteriol. 76: 79-85. [18] Jayasree, L., Janakiram, P. and Madhavi, R. (2006) Characterization of Vibrio spp. associated with diseased shrimp from culture ponds of Andhra Pradesh (India). J. World Aquac. Soc. 37: 523-532, https://doi.org/10.1111/j.1749-7345.2006.00066.x [19] Alagappan, K.M., Deivasigamanim, B., Somasundaramm, S.T. and Kumaran, S. (2010) Occurrence of Vibrio parahaemolyticus and Its Specific Phages from Shrimp Ponds in East Coast of India. Curr. Microbiol. 61: 235-240, https://doi.org/10.1007/s00284-010-9599-0 [20] Jiravanichpaisal, P. and Miyazaki, T. (1994) Histopathology, biochemistry and pathogenicity of Vibrio harveyi infecting black tiger shrimp Penaeus monodon. J. Aquat. An. Health 6: 27-35, https://doi.org/10.1577/15488667(1994)006%3C0027:HBAPOV%3E2.3. CO;2 [21] Lavilla-Pitogo, C.R., Leano, E.M. and Paner, M.G. (1996) Mortalities of pond cultured juvenile shrimp, Penaeus monodon, associated with dominance of luminescent bacteria, Vibrio harveyi in the rearing environment. SICCPPS book of abstracts, SEAFDEC, Iloilo City, Philippine, 40. [22] Corliss, J., Lightner, D.V. and Zein-Eldin, P. (1977) Some effects of oral doses of oxytetracycline on growth, survival and disease in Penaeus aztecus. Aquaculture 11: 355–362, https://doi.org/10.1016/0044-8486(77)90085-0 [23] Mohney, L., Bell, T.A. and Lightner, D.V. (1992) Shrimp antimicrobial testing-1. In vitro susceptibility of thirteen gram negative bacteria to twelve antibiotics. Journal of Aquatic Animal Health 4: 257–261, https://doi.org/10.1577/1548-8667(1992)004%3C0257:SATIIV%3E2.3.CO;2 [24] Ruangpan, L. and Kitao, T. (1991) Vibrio bacteria isolated from black tiger shrimp, Penaeus monodon Fabricius. Journal of Fish diseases 14: 383-388, https://doi.org/10.1111/j.1365-2761.1991.tb00836.x [25] Charantchakool, P., Pearson, M., Limsuwan, C. and Roberts, R.J. (1995) Oxytetracycline sensitivity of Vibrio spp. isolated from diseased black tiger shrimp, Penaeus monodon Fabricius. Journal of Fish Diseases 18: 79–82, https://doi.org/10.1111/j.1365-2761.1995.tb01268.x [26] Lee, S.W., Najiah, M., Wendy, W. and Nadirah, M. (2009a) Comparative study on antibiogram of Vibrio spp. isolated from diseased postlarval and marketable-sized white leg shrimp (Litopenaeus vannamei). Front. Agric. China 3: 446-451, DOI: 10.1007/s11703-009-0068-0 [27] Razvykh, V.M., Friauf, E.V., Givental, N.I., Bogdanova, L.F. and Vedmina, E.A. (1990) Atibiotic sensitivity of Vibrio Parahaemolyticus isolated in the turkmene SSR. Antibiot. Khimioter. 35: 23-24. [28] Abraham, T.J., Manley, R., Papanippan, R. and Dhevendran, K. (1997) Pathogenicity and antibiotic sensitivity of luminous Vibrio harveyi isolated from diseased penaeid shrimp. Journal of Aquaculture in Tropics 12: 1–8. [29] Thakur, A.B., Vaidya, R.B. and Suryawanshi, S.A. (2003) Pathogenicity and antibiotic susceptibility of Vibrio species isolated from moribund shrimps. India J. Mar. Sci., 32: 71-75. [30] Herwig, R.P. and Gray, J.P. (1997) Microbial response to antibacterial treatment in marine microcosms. Aquaculture 152: 139-154, https://doi.org/10.1016/S0044-8486(96)01517-7 [31] Karunasagar, I., Pai, R., Malathi, G.R. and Karunasagar, I. (1994) Mass mortalities of Penaeus monodon larvae due to antibiotic resistant Vibrio harveyi infection. Aquaculture 128: 203–209, https://doi.org/10.1016/0044-8486(94)90309-3 [32] Uddin, S.A. and Kader, M.A. (2006) The use of antibiotics in shrimp hatcheries in Bangladesh. J. Fisheries and Aquatic Sciences 1(1): 64-67, DOI: 10.3923/jfas.2006.64.67 [33] Graslund, S. and Bengtsson, B.E., (2001) Chemicals and biological products used in south-east Asian shrimp farming and their potential impact on the environment– a review. The Science of the Total Environment 280: 93-131, DOI: 10.1016/s0048-9697(01)00818-x