A Simple Procedure for Extraction of Surface Protein of Salmonella Serotypes and Escherichia coli Strains Isolated from Poultry and Pigs

Salmonella and E.coli are common bacteria in intestinal tract that is responsible for a variety of intestinal disorders in swine and poultry . The expression of E.coli and Salmonella virulence genes causes gastrointestinal diseases in animals as well as intoxications in humans using the meat contamination bacteria . In order to reduce the intensive intestinal colonisation of pathogenic Salmonella and E.coli in commercial farms, various strategies have been applied, such as antibiotics supplementation in the diet or supplementation of additives that inhibit bacterial adhesion to the intestinal epithelium, competitive exclusion by non-pathogenic bacteria, genetic selection of animal strains, and development of vaccines . 
 

Enterobacteriaceae possesses different surface structures (proteins or antigens) that can induce protective immune responses . The utilization of an optimized method for surface protein extraction should greatly enable applications for the therapy and diagnosis including the production of ELISA kit and subunit vaccine as well as other proteomic research. Nowadays, the protein extraction from the bacteria surface was done by many methods such as the cell surface protein isolation kit, the use of a rotating cylinder probe or a combination of detergent and phase separation using Triton X-114 (TX-114) . However, most of these methods have limitations due to augmentation of performance duration, the expense as well as special materials in laboratory.

The aim of this study is to determine a simple extraction procedure for obtaining the surface protein complexes of Salmonella serotypes and E.coli strains. This is the first step to identify immune-reactive proteins in the next approach. Therefore, identification of protein components obtaining from extraction was carried out by examining the molecular weight of proteins separated in SDS-PAGE bands profile.





DOI: https://doi.org/10.30564/vsr.v1i2.1357