Selection of High-quality Sperms by the Nanotechnological Method of Magnetic Activation in Brazilian Cervids

Source:
By:Teresinha Inês de Assumpção, André Luís Quagliatto Santos, José Maurício Barbanti Duarte, Caroline Silva Vieira

DOI: https://doi.org/10.30564/jzr.v3i2.3106

Abstract:

Cervids show a high degree of abnormalities in their sperm cells. Thus, this study aimed to select high-quality spermatozoa using magnetic-activated sperm sorting (MASS) compared to density gradient centrifugation (DGC) by assessing the post-selection cell quality. Semen from six Mazama deer was collected by electroejaculation after chemical restraint. The semen was analyzed in four samples: Fresh, DGC, SEMgood - non-apoptotic fraction,and SEMpoor - apoptotic fraction. The material was analyzed for motility and vigor (light microscopy), concentration (Neubauer chamber), semen morphology (phase contrast), and supravital staining test (eosin/ nigrosine).The DGC method used 20 x 106 cells in 90% and 45% percoll® gradient.The MASS used 10 x 106 cells with 20 μl of iron nanoparticles attached to Annexin V and filtration in a magnetic separation column. Both processing methods (DGC and MASS) were effective in producing high-quality spermsamples, with a marked reduction in abnormalities from 41.83 ± 10.25 (fresh) to 14.83 ± 3.17(DGC) and 12 ± 3.01 (SEMgood), with 80.3% ± 2.06 livings cells. These findings suggest that this nanotechnological method,using nanoparticles, effectively produces high-quality semen samples in cervids for use in assisted reproduction.

References:

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