Food Habits and Helminth Parasites of Skittering Frog (Euphlyctis cyanophlyctis) in the Lower Dir, Pakistan

Updata:01-01-1970

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By:Anwar Ullah, Mohammad Attaullah, Wali Khan, Abdul Waris, Shah Khalid, Abdul Baset

DOI: https://doi.org/10.30564/jzr.v2i4.2268

Abstract:
A total of 30 specimens of Skittering frog (Euphlyctis cyanophlyctis),
(Anura: Dicroglossidae) were captured from seven selected localities in
the Dir Lower, Khyber Pakhtunkhwa province of Pakistan from April
to August 2016. They were euthanized with the help of Chloroform
(CHcl3) solution. The biometry of each specimen was measured, then
dissected to examine for helminth parasites and foodstuff. All helminths were isolated, washed with normal saline at room temperature,
and then preserved in 70% GL ethyl alcohol. Later on, the parasites
were classified and studied under the electric compound microscope. In
a total of 30 specimens, 17(56.66%) were found infected with 62 helminths parasites belongs to four different species. Among four species,
one was Cestodes Nematotaenia dispar species and remaining three
were Nematodes i.e. Cosmocerca species, Cosmocercoides (Raillietnema species) and Strongyloides species. Cosmocerca with a percentage
of 56.45% was the most dominant. In the total of 30 specimens’ alimentary canal, 10 were found empty, containing nothing while in remaining
20 were full of 9 types of food materials which included insects i.e.
Ants, Beetles, Wasps, Spiders, Mosquito larvae and cockroaches, and
non-living things like stone pebbles and mollusks shells. Among the serial, flies and snails were in the lowest prevalence and ants were recorded the most important component of the frog diet. Among the insects,
ants have occurred in higher prevalence 44.50% and flies 1.15% with
the lowest prevalence. The results emphasize the significance of further
expected studies in the Dir Lower to get a good understanding of the
helminth parasites with the ecological relations of their hosts.
References:

[1] Vitt, L. J., Caldwell, J. P. Herpetology: An Introductory Biology of Amphibians and Reptiles. 4ª Edição, 2014. [2] Borroto-Páez, R., Bosch, R. A., Fabres, B. A., García, O. A. Introduced amphibians and reptiles in the Cuban archipelago. Herpetological Conservation and Biology, 2015, 10(3): 985-1012. [3] Wake, D. B., Koo, M. S. Amphibians. Current Biology, 2018, 28(21): R1237-R1241. [4] Sarwar, M. K., Malik, M. F., Hussain, M., Azam, I., Ashiq, U. Distribution and current status of amphibian fauna of Pakistan: A review. Electronic Journal of Biology, 2016, 12(3): 243-246. [5] Ali, W., Javid, A., Hussain, A., Bukhari, S. M. Diversity and habitat preferences of amphibians and reptiles in Pakistan: a review. Journal of Asia-Pacific Biodiversity, 2018, 11(2): 173-187. [6] Wright, L. The looming tower: Al-Qaeda and the road to 9/11. Alfred a Knopf Incorporated, 2006. [7] Rataj, A. V., Lindtner-Knific, R., Vlahović, K., Mavri, U., Dovč, A. Parasites in pet reptiles. Acta Veterinaria Scandinavica, 2011, 53(1): 33. [8] Bursey, C. R., Goldberg, S. R., Parmelee, J. R. Gastrointestinal helminths of 51 species of anurans from Reserva Cuzco Amazónico, Peru. Comparative Parasitology, 2001, 68(1): 21-35. [9] Malan, K. Taxonomy and ecology of parasitic chigger mites (Acari: Trombiculidae) on small mammals in South Africa (Doctoral dissertation, Stellenbosch: Stellenbosch University), 2015. [10] Akani, G. C., Luiselli, L., Amuzie, C. C., Wokem, G. N. Helminth community structure and diet of three Afrotropical anuran species: a test of the interactive-versus-isolationist parasite communities hypothesis. Web ecology, 2011, 11(1), 11-19. [11] Campiao, K. M., de Aquino Ribas, A. C., Morais, D. H., da Silva, R. J., Tavares, L. E. R. How many parasites species a frog might have? Determinants of parasite diversity in South American anurans. PLOS one, 2015, 10(10): e0140577. [12] Johnson, P. T., McKenzie, V. J. Effects of environmental change on helminth infections in amphibians: exploring the emergence of Ribeiroia and Echinostoma infections in North America. In The biology of echinostomes. Springer, New York, NY, 2009: 249-280. [13] Behringer, D. C., Karvonen, A., Bojko, J. Parasite avoidance behaviours in aquatic environments. Philosophical Transactions of the Royal Society B: Biological Sciences, 2018, 373(1751), 20170202. [14]. Imkongwapang, R., Jyrwa, D. B., Lal, P., Tandon, V. A checklist of helminth parasite fauna in anuran Amphibia (frogs) of Nagaland, Northeast India. Journal of parasitic diseases, 2014, 38(1): 85-100. [15] Akbar Hussain, M.A., Rafi, M.A., Khan, H., Waris, A., Zeb, A., Baset, A. Diversity of carpenter bee fauna (Xylocopa Spp.) In DIR lower, khyber pakhtunkhwa, pakistan., Indo Am. J. P. Sci, 2019, 06(10). [16] Khalid, S., Attaullah, M., Waris, A., Baset, A., Masroor, R., Khan, A.U., Khan, I. 2019. Diversity and distribution of lizard fauna in tehsil Samar Bagh, Dir lower, khyber Pakhtunkhwa, Pakistan. International Journal of Fauna and Biological Studies, 2019, 6(6): 20-25. [17] Boulenger, G. A. The tailless batrachians of Europe (Vol. 74). Ray Society, 1897. [18] Tsuji, M., Ushimaru, A., Osawa, T., Mitsuhashi, H. Paddy-associated frog declines via urbanization: A test of the dispersal-dependent-decline hypothesis. Landscape and Urban Planning, 2011, 103(3-4): 318-325. [19] Jabon, K. J., Gamalo, L. E., Responte, M., Abad, R., Gementiza, G. D., Achondo, M. J. M. Density and diet of invasive alien anuran species in a disturbed landscape: A case in the University of the Philippines Mindanao, Davao City, Philippines. Biodiversitas Journal of Biological Diversity, 2019, 20(9). [20] Mohammad, M. K., Al-Moussawi, A. A., Jasim, S. Y. Helminth parasites of the green toad Bufo viridis Laurenti, 1768 in Baghdad area, central Iraq. Egyptian Academic Journal of Biological Sciences, B. Zoology, 2010, 2(1): 17-25. [21] McALPINE, D. F., Burt, M. D. Helminths of Bullfrogs, Rana catesbeiana, Green Frogs, R. clamitans, and Leopard Frogs, R. pipiens, in New Brunswick. Canadian Field Naturalist, 1998, 112(1): 50-68. [22] KARAKAŞ, M. Helminth Parasites of Bufo viridis, Rana ridibunda and Hyla arborea Collected from the Different Regions of Turkey. Manas Journal of Agriculture Veterinary and Life Sciences, 2015, 5(1). [23] Ruiz-Torres, N., García-Prieto, L., Lagunas-Calvo, O., Violante-González, J., Osorio-Sarabia, D. Helminth infracommunity of the cane toad Rhinella marina (Anura: Bufonidae) within its native distribution range. Parasitology international, 2017, 66(5): 567-572. [24] Daly, J. W., Garraffo, H. M., Spande, T. F., Giddings, L. A., Saporito, R. A., Vieites, D. R., Vences, M. Individual and geographic variation of skin alkaloids in three species of Madagascan poison frogs (Mantella). Journal of chemical ecology, 2008, 34(2): 252-279. [25] Sulieman, Y., Afifi, A., Awad, H. M., Pengsakul, T. Helminth parasites of the subdesert toad, Amietophrynus (Bufo) xeros (Anura: Bufonidae). International Journal of Research–Granthaalayah, 2015, 3(10): 75-83. [26] Yildirimhan, H. S., Bursey, C. R., Goldberg, S. R. Helminth parasites of the Caucasian salamander, Mertensiella caucasica, from Turkey. Comparative Parasitology, 2005, 72(1): 75-87. [27] AL-SORAKHY MK, AMR ZS. Platyhelminth parasites of some amphibians in Jordan. Turkish Journal of Zoology, 2003, 27(2):89-93. [28] Biserkov, V.Y., Yildirimhan, H.S., Buchvarov, G. and U¤urtafl, I.H. Polystoma macrocnemis n. sp. (Monogenea: Polystomatidae) from the Iranian longlegged wood frog Rana macrocnemis (Ranidae) in Turkey. Systematic Parasitology, 2001, 48 (1): 61-66. [29] Al-khamesi mb, Salman is, Abid sa, Ibrahim sm. Study prevalence and effect of internal parasites and histopathological changes on common frogs at baghdad city. The Iraqi Journal of Agricultural Science, 2018, 49(3): 445-51. [30] Iyaji FO, Medayedupin IT, Echi PC, Falola OO, Omowaye OS. Gastrointestinal helminth parasites of Amietophyrnus regularis, Bufonidae (African common toad) in Anyigba, Kogi State, Nigeria. Animal Research International, 2015, 12(2): 2231-40. [31] Jablonski D, Jandzik D, GVOŽDÍK V. New records and zoogeographic classification of amphibians and reptiles from Bosnia and Herzegovina. North-Western journal of zoology, 2012, 8(2).

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